Visualizing the ultra-structure of microorganisms using table-top extreme ultraviolet imaging

Chang Liu^{1, 2, 3},
Wilhelm Eschen^{1, 2, 3},
Lars Loetgering^{1, 2, 3, 4},
Daniel S. Penagos Molina^{1, 2, 3},
Robert Klas^{1, 2, 3},
Alexander Iliou⁵,
Michael Steinert¹,
Sebastian Herkersdorf⁶,
Alexander Kirsche^{1, 2, 3},
Thomas Pertsch^{1, 7},
Falk Hillmann^{5, 8},
Jens Limpert^{1, 2, 3, 7},
Jan Rothhardt^{1, 2, 3, 7}

1.
Institute of Applied Physics, Abbe Center of Photonics, Friedrich-Schiller-University Jena, Albert- Einstein-Straße 15, 07745, Jena, Germany
2.
Helmholtz-Institute Jena, Fröbelstieg 3, 07743, Jena, Germany
3.
GSI Helmholtzzentrum für Schwerionenforschung, Planckstraße 1, 64291, Darmstadt, Germany
4.
Present address: Carl Zeiss AG, Carl-Zeiss-Promenade 10, 07745, Jena, Germany
5.
Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute (Leibniz- HKI), Adolf-Reichwein-Str
6.
Department of Pharmaceutical Microbiology, Hans Knöll Institute (HKI), Friedrich-Schiller- University Jena, Winzerlaer Straße 2, 07745, Jena, Germany
7.
Fraunhofer Institute for Applied Optics and Precision Engineering, Albert-Einstein-Str
8.
Present address: Biochemistry/Biotechnology, Faculty of Engineering, Hochschule Wismar University of Applied Sciences Technology, Business and Design, Philipp-Müller-Str

Funds: We thank Dirk Hoffmeister for his help with a fruitful contribution to the manuscript.

摘要

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Abstract: Table-top extreme ultraviolet (EUV) microscopy offers unique opportunities for label-free investigation of biological samples. Here, we demonstrate ptychographic EUV imaging of two dried, unstained model specimens: germlings of a fungus (Aspergillus nidulans), and bacteria (Escherichia coli) cells at 13.5 nm wavelength. We find that the EUV spectral region, which to date has not received much attention for biological imaging, offers sufficient penetration depths for the identification of intracellular features. By implementing a position-correlated ptychography approach, we demonstrate a millimeter-squared field of view enabled by infrared illumination combined with sub-60 nm spatial resolution achieved with EUV illumination on selected regions of interest. The strong element contrast at 13.5 nm wavelength enables the identification of the nanoscale material composition inside the specimens. Our work will advance and facilitate EUV imaging applications and enable further possibilities in life science.
- EUV /
- Ptychography /
- Microorganism /
- HHG

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参考文献(61)

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