Cellular-resolution in vivo tomography in turbid tissue through digital aberration correction

En Bo; Xin Ge; Yuemei Luo; Xuan Wu; Si Chen; Haitao Liang; Shufen Chen; Xiaojun Yu; Ping Shum; Jianhua Mo; Nanguang Chen; Linbo Liu

doi:10.1186/s43074-020-00009-7

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En Bo, Xin Ge, Yuemei Luo, Xuan Wu, Si Chen, Haitao Liang, Shufen Chen, Xiaojun Yu, Ping Shum, Jianhua Mo, Nanguang Chen, Linbo Liu. Cellular-resolution in vivo tomography in turbid tissue through digital aberration correction[J]. PhotoniX. doi: 10.1186/s43074-020-00009-7

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Cellular-resolution in vivo tomography in turbid tissue through digital aberration correction

doi: 10.1186/s43074-020-00009-7

1.
School of Electrical and Electronic Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Republic of Singapore
2.
School of Automation, Northwestern Polytechnical University, Xi’an, 710072, Shaanxi, China
3.
School of Electronics and Information Engineering, Soochow University, Shizi Street 1, Suzhou, 215006, China
4.
Department of Biomedical Engineering, National University of Singapore, Singapore, 117583, Republic of Singapore
5.
School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, Singapore, 637459, Republic of Singapore

Funds:

This research was supported by National Research Foundation Singapore under its Competitive Research Program (NRF-CRP13-2014-05), Ministry of Education Singapore under its Academic Research Fund Tier 1 (2018-T1-001-144), and Agency for Science, Technology and Research (A^*STAR) under its Industrial Alignment Fund (Pre-positioning) (H17/01/a0/008).

Received Date: 2019-07-26
Accepted Date: 2020-02-20

Available Online: 2020-03-12

Abstract

Abstract

Noninvasive tomographic imaging of cellular processes in vivo may provide valuable cytological and histological information for disease diagnosis. However, such strategies are usually hampered by optical aberrations caused by the imaging system and tissue turbidity. State-of-the-art aberration correction methods require that the light signal be phase stable over the full-field data acquisition period, which is difficult to maintain during dynamic cellular processes in vivo. Here we show that any optical aberrations in the path length difference (OPD) domain can be corrected without the phase stability requirement based on maximum intensity assumption. Specifically, we demonstrate a novel optical tomographic technique, termed amplitude division aperture synthesis optical coherence tomography (ADAS-OCT), which corrects aberrations induced by turbid tissues by physical aperture synthesis and simultaneously data acquisition from sub-apertures. Even with just two sub-apertures, ADAS-OCT enabled in vivo visualization of red blood cells in human labial mucosa. We further demonstrated that adding sub-apertures could significantly scale up the aberration correction capability. This technology has the potential to impact a number of clinical areas where noninvasive examinations are preferred, such as blood count and cancers detection.
- Turbid tissue,
- Tomography,
- Aberration correction,
- Cellular resolution

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References(34)

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